This study aims to deepen the understanding of processes that affect collaboration between professions and organisations in the crisis preparedness domain from a gender perspective. A total of twenty-three Swedish duty officers participated in the study. The analysis of the interviews show that collaboration can be understood as (a) the militarisation of civil crisis management actors, which means that many of the work processes and cultures that originate in military organisations can now be found in the security and crisis management sector; (b) the masculinisation, which means that when male dominance appears to prevail, active strategies are used against women, civilian personnel and also inexperienced colleagues and (c) organisational exclusion which emerges particularly in situations where collaboration between female-dominated and male-dominated organisations are required. The findings are important for crisis preparedness research and practice and should work in favour of evening out asymmetries in collaborative crisis management.